Microbiology Collections

Urinary Tract

Urine, voided
Preparation Various mild antiseptics around urethra.
Type, Number or Volume Clean, mid-stream collection. 10ml minimum.
Container or Transport Device Sterile screw cap container.
Technique Hold the labia apart or the foreskin retracted.
Comments See Midstream (clean catch) urine collection instructions.
Urine, catheterized
(or ileal loop)
Preparation Clamp drainage tubing. Cleanse tubing with alcohol.
Type, Number or Volume 1ml minimum.
Container or Transport Device Sterile screw cap container.
Technique Aspirate through port with syringe 10-20ml. Special devices for obtaining uncontaminated ileal loop cultures.
Comments Suprapubic aspiration may be necessary.
Bladder urine
(suprapubic or cystoscopic)
Preparation Cleanse skin prior to aspiration. Indicated in patients with clinical evidence of UTI where collection of clean catch is difficult or not appropriate, neonates, young infants suspected anaerobic infection.
Type, Number or Volume 1ml minimum.
Container or Transport Device Sterile screw cap container.
Technique Collected by physician by needle aspiration or cystoscopy.
Comments Anaerobic culture may be of value but is not routinely done. Contact Microbiology laboratory for details.