| BRONCHOSCOPY |
| Preparation |
- |
| Type, Number or Volume |
Brushings, trans-bronchial biopsies, secretions. |
| Container or Transport Device |
Sterile screw top container. |
| Technique |
Aspirated through inner chamber of bronchoscope. Collected by physician. |
| Comments |
Possible contamination with oropharyngeal organisms during instrument passage. |
| EXPECTORATED SPUTUM |
| Preparation |
May require induction by nebulizer, hydration, physiotherapy, or postural drainage; may require patient to brush teeth and rinse before specimen is taken. |
| Type, Number or Volume |
Sputum (not saliva) |
| Container or Transport Device |
Sterile screw top container. |
| Technique |
Best taken under supervision to help induce deep cough. |
| Comments |
Screened by Gram stain prior to culture. May be refrigerated. Sputum cultures are not helpful in absence of respiratory symptoms or signs. |
| For Mycobacterium |
| Preparation |
For Mycobacterium-3 early morning, fresh specimens from deep cough. |
| Type, Number or Volume |
5-10 ml (3 ml minimum) |
| Container or Transport Device |
Sterile screw top container, placed in sealed biohazard bag. |
| Technique |
- |
| Comments |
- |
| TRACHEAL ASPIRATE |
| Preparation |
Aseptic technique. |
| Type, Number or Volume |
Sputum. |
| Container or Transport Device |
Sterile container. |
| Technique |
Use sterile suction catheter to aspirate from tracheostomy or endotracheal tubes. |
| Comments |
May be refrigerated. |