Specialized Testing

Cortisol (ACTH) Stimulation

Adrenocorticotropic Hormone (ACTH) Stimulation

Order Name: ACTH STIM
Test Number: 2002151
Test Description

This test determines how the adrenal glands respond to a certain hormone. The hormone is called adrenocorticotrophic hormone, also known as ACTH. ACTH is made in the pituitary gland and travels through the bloodstream to the adrenal glands. ACTH stimulates the adrenal gland to release Cortisol. Cortisol is a key factor in many functions in the body's metabolism of fats, carbohydrates, sodium, potassium, and protein.

For collection information refer to this test in the Test Directory.

Testing Locations

Cortisol (ACTH) Stimulations may be scheduled with RML's PSC center at St. John Medical Center for collections M-F 0730am to 4:00pm at (918) 744-3131 x 15531; after 4:00pm may be scheduled with Chemistry department at 981-744-3131 x 15514. Testing is performed Monday through Friday 9:00am to 11am. and requires about 1 hour and 15 minutes of the patient's time.


Cortisol (ACTH) stimulations may be scheduled with RML Laboratory at Jane Phillips Medical Center, Monday through Friday at (918) 331-1737.

Physician Office

If the testing is performed in the physician's office, the following protocol is suggested.

ACTH Stimulation Test
  1. Baseline Cortisol - Have a Cortisol drawn prior to injection (5 ml gold top tube). Note the time drawn on the tube.
  2. Administer 250 micrograms (1 vial) of synthetic ACTH Cortrosyn (Cosyntropin) intravenously. Use supplied diluents to dissolve ACTH powder. Inject over 1-2 minutes.
  3. 30 minute Cortisol - draw one clot tube for a Cortisol level 30 minutes after the injection. Note the time drawn on the tube.
  4. 60 minute Cortisol - draw a second clot tube for a Cortisol level 60 minutes after the injection. Note the time drawn on the tube.